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Cryopreservation of Human Semen

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Cryopreservation of Human Semen | SpringerLink

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Human Sperm Cryopreservation

To investigate whether the sperm fertilizing potential can be improved by selecting a non-apoptotic fraction using magnetic activated cell sorting MACS , and to compare the results with the conventional swim-up method. Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Relative contributions of unfrozen water and salt concentration to the survival of slowly frozen human erythrocytes. This can potentially increase the risk of mutations in offspring DNA.
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